Question

What should the cell concentration be for the sorting?

Answer

• If there are too few cells, sorting will take longer and cell viability may be affected.
• If the cells are too concentrated, it may reduce purity and cause instrument clogging.
  👉 An optimal cell preparation improves viability and leads to higher yield.

Guidelines:

• If you have fewer than 10×10⁶ cells, prepare them in a volume of 0.5 mL.
• The recommended concentration for the BD FACSAria III is 20–30 million/mL, depending on cell type.
• If the sample is too concentrated, we can dilute it at the facility.
• If unsure, it’s better to bring the sample more concentrated (it can always be diluted).
• Count the cells right before sorting (after all washes).