Question

How large can the cells be?

Answer

The BD FACSAria III is a cell sorter equipped with 70, 85, 100, and 130 μm nozzles. In general, the diameter of the cells to be sorted should be at least five times smaller than the nozzle size. The FACSAria III also provides three neutral density filters (1.0, 1.5, 2.0) to better define the forward scatter based on the morphological characteristics of the cells being sorted.