Question

Are there ways to improve sorting?

Answer

• Use polypropylene tubes for cell preparation and sorting.
• Use polypropylene collection tubes and fill them (about 1/2 mL) with medium containing 30% serum (for eukaryotic cells).
• Count the cells immediately before sorting (after all washes).
• Use the “Yield” sorting mode to improve recovery (Note: purity will decrease!).
• Process collection tubes immediately after filling or at the end of sorting.