Question

Will my cells be damaged during the sorting?

Answer

It’s important to note that the toxicity of pre-sort staining can affect cell viability and it should be evaluated before the sorting experiment.
In general, cells may experience mechanical stress during the process, but this can be minimized by keeping them at the appropriate temperature, pH, and in the most suitable medium.

The BD FACSAria III is equipped with a 100 μm nozzle, which can be operated at lower pressure compared to smaller nozzles, automatically reducing mechanical stress.
Additionally, the user can set the temperature of the collection chamber, helping to maintain optimal conditions for the cells.