Spectroscopy
"The truth is always found in simplicity, never in confusion." - Isaac Newton
Description
The Spectroscopy Laboratory offers its services within the field of biochemical studies. It is equipped with a Spectropolarimeter and a Spectrofluorimeter.
The Spectropolarimeter is designed for analyzing the structural properties of biomolecules and optically active compounds, for determining the secondary structure of proteins (α-helix, β-sheet, random coil) and for studying conformational changes in proteins during interactions with substances (inhibitors, substrate analogs, etc.) or as a result of them (e.g., denaturing agents).
The Spectrofluorimeter is used to study the light emitted by a sample when excited by a source of ultraviolet or visible light. The analysis of fluorescence emission spectra allows for the identification and quantification of fluorescent molecules present in a sample.
Equipment
Software
| Name | Description | |
|---|---|---|
| Spectra Manager II™ | is an innovative cross-platform molecular spectroscopy software. This single solution for data analysis is a unique and powerful way to measure, process, display and report data from any JASCO spectroscopy system including UV-Visible/NIR, Fluorescence, FTIR, Circular Dichroism, ORD, LD, Raman, Polarimeters. The single intuitive user-interface is found across the entire range of instruments eliminating the need to learn multiple software packages. | link |
| FL Winlab™ | is a software for analyzing data acquired with Perkin Elmer fluorescence-based instruments. |
Rates
The Centre’s regulations provide for the application of a fee for the use of the equipment:
INTERNAL USERS
Consultation is reserved for University staff: link
EXTERNAL USERS
| Equipment | Autonomous use | With technical support |
|---|---|---|
| PERKIN ELMER LS50B Spectrofluorimeter (hourly rate) | € 6,00 | not available |
| JASCO J-1500 Spectropolarimeter (daily rate) | € 30,00 | not available |
