Question

What types of tubes are used for cell collection?

Answer

The BD FACSAria III can sort up to four cell populations simultaneously (4-way sorting), using 5 mL FACS tubes (sterile, polypropylene, round-bottom, 12×75 mm), 15 mL conical Falcon tubes, or 1.5 mL Eppendorf tubes.

Please bring labeled collection tubes containing about 1/2 mL of cell culture medium.

Sterile 5 mL polypropylene FACS tubes are also required for post-sort acquisition controls.

Additionally, the BD FACSAria III is equipped with a holder for single-cell collection into 96- (and 384-) well plates. Plates should be brought pre-filled with medium.