X500B QTOF Quadrupole/Time of Flight (Sciex) is a high-performance UHPLC-HRMS/MS system.

Features include: mass range 1–40 kDa, MS/MS scan speed up to 100 Hz, resolution ≥ 42,000 (FWHM), MS/MS sensitivity of 1.0 pg of reserpine on column, accuracy < 2 ppm, Turbo V source with Twin Sprayer ESI/APCI Probe, acquisition modes in Full Scan, targeted (IDA) and untargeted (SWATH).

Data acquisition software: Sciex™ SCIEX OS.

This system is used for high-resolution accurate mass analysis, for the characterization of biomolecules (metabolomics, bottom-up and top-down proteomics, lipidomics) and for the identification and quantification of molecules in different biological matrices and plant extracts.

Available methods:

• High-resolution (HRMS) analysis of ionizable compounds in solution by direct infusion, providing exact mass for elemental formula confirmation;
• Qualitative/quantitative analysis of small molecules/biomarkers;
• Development of UHPLC-HRMS/MS methods for the identification and quantification of organic, biorganic, biopharmaceutical and organometallic compounds that are soluble and ionizable, in mixtures and complex matrices;
• Targeted and untargeted metabolomics studies;
• Structural characterization of peptides (Peptide Mapping);
• Intact protein analysis;
• Native protein analysis;
• Membrane lipid analysis;
• Monoclonal antibody analysis;
• Pharmacokinetic studies.

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