Mass Spectrometry
“I think the hard-core chemists saw it as kind of an unexplainable, voodoo, black magic kind of a tool”. László Tőkés, “The allure of mass spectrometry: From an early-day chemist's perspective”, 2017
Description
A mass spectrometry platform for the analysis of small and large molecules from purified samples or complex matrices, suitable for identification, quantification, structural characterization and “omics” studies with very high resolution.
The platform offers support and assistance to users starting from method development to qualitative and quantitative data analysis. The instrumentation is also accessible for autonomous use after training.
Custom analysis packages can be arranged for the development of personalized identification and quantification methods.
Additionally, two dedicated workstations are available exclusively for data analysis and reprocessing.
Equipment
Software
| Name | Description | |
|---|---|---|
| Xcalibur | Xcalibur™ is Thermo Scientific™’s standard software for MS data acquisition and processing through a single interface, enabling method development, data acquisition and processing and report creation. | link |
| FreeStyle | Software included in the previous, for qualitative analysis of mass spectrometry data in .raw format; available for free download. | link |
| SciexOS | SciexOS is Sciex™`s standard software for data acquisition and processing based on the Windows® operating system, featuring multiple viewing windows to simplify data processing and acquisition. | link |
| MaxQuant | MaxQuant is an Open-source quantitative proteomics software package designed for the analysis of large high-resolution mass spectrometry datasets. | link |
Rates
The Centre’s regulations provide for the application of a fee for the use of the equipment:
INTERNAL USERS
Consultation is reserved for University staff: link
EXTERNAL USERS
| Equipment/Service | Autonomous use (hourly rate) | With technical support (hourly rate) |
|---|---|---|
| DSQ-Pal, DSQII, (GC-MS) | not available | € 80,00 |
| TSQ Quantum, (GC-MS) | not available | € 80,00 |
| LCQ Fleet (Analisi HPLC-ESI/APCI-MS) | not available | € 100,00 |
| HR-MS AB Sciex X500B | not available | € 120,00 |
| Special treatments of the sample, organization and treatment of data, drafting of reports | not available | € 100,00 |
EXTERNAL USERS WITH AGREEMENTS
| Equipment/Service | Autonomous use (hourly rate) | With technical support (hourly rate) |
|---|---|---|
| DSQ-Pal, DSQII, (GC-MS) | not available | € 36,00 |
| TSQ Quantum, (GC-MS) | not available | € 36,00 |
| LCQ Fleet (Analisi HPLC-ESI/APCI-MS) | not available | € 43,20 |
| HR-MS AB Sciex X500B | not available | € 84,00 |
| Special treatments of the sample, organization and treatment of data, drafting of reports | not available | € 30,00 |
