Question
Which fluorochromes can be analysed?
Answer
It is necessary to verify in advance whether the fluorescent proteins or fluorochromes used can be detected by the cytometer.
Not all fluorochromes visible under the microscope can also be measured by the cytometer.
To check if the optical configuration is suitable, online tools called Spectrum Viewers are available from various companies, for example:
- https://www.bdbiosciences.com/us/s/spectrumviewer
- https://www.thermofisher.com/us/en/home/life-science/cell-analysis/labeling-chemistry/fluorescence-spectra-viewer.html
The configuration of the BD FACSAria III is provided in the Equipment section.