Transmission Electron Microscopy TEM

 Massimo Boiocchi
tel. 0382987531  massimo.boiocchi@unipv.it

The technique

Transmission electron microscopy (TEM) allows to obtain very magnified images of specimens properly prepared. Images are produced by an electron beam travelling through the specimen and focused onto a photographic film or a digital detector (CCD camera). The transmission electron microscope Jeol JEM-1200EX II is used to observe organic and inorganic nanoparticles, finely dispersed on a TEM grid, as wheel as ultrathin section (<100 nm) of biological samples (cells or tissues), cut with a microtome. The laboratory staff help users in the use of the microscope whereas requests of assistance for sample preparation have to be evaluated individually.

Equipment

  • JEOL JEM-1200EX II transmission electron microscope equipped with the TEM CCD camera Mega View III
  • Ultramicrotome Reichert Ultracut S