Put 6 g glycerol in a 50 ml plastic centrifuge tube and add a small stir bar. (note: grams not ml)
Add 2.4 g Mowiol (Calbiochem); stir to mix
While stirring, add 6 ml distilled water and leave 2 hours at room temperature
Add 12 ml 0.2M Tris (pH 8.5)
OPTIONAL: Add a small amount of NaN3 (Sodium Azide) such that the final concentration is .02%
Incubate the tube in hot water (50 – 60ºC) for 10 min to dissolve the Mowiol. This can be repeated over several hours if necessary
Add Dabco (Sigma D27802) for a final 2.5% (w/v; 2,5g/100ml) and vortex until dissolved
Centrifuge at 5000 g for 15 minutes to remove any undissolved solids. Store small aliquots in Eppendorf tubes at -20ºC
Warm tubes to room temperature for use. Opened tubes can be stored at 4ºC for approximately 1 week. Discard if any crystalline material is seen in the tube or on the slides
Leave coverslipped slides in the dark overnight to harden before oil immersion lenses are used
