Barbara Mannucci
tel. 0382987532 barbara.mannucci@unipv.it
Mass spectrometry is a powerful analytical technique that is used to identify unknown compounds, to quantify known compounds and to elucidate the structure and chemical properties of molecules. Mass spectrometry provides valuable information in a wide range of areas: organic and organometallic molecules are the most analyzed. Detection of compounds can be accomplished with very minute quantities and compounds can be identified at very low concentrations in chemically complex mixtures. These features get mass spectrometry useful in different areas: Environmental (organic pollutants in water, soil, food), Pharmaceutical (identification and quantification of degradation products and impurities in drugs, metabolic products of drugs) Cosmetic (determination of natural allergens in cosmetics and natural extracts) Biotechnology (analysis of proteins and peptides) Forensic medicine (determination of drugs in biological fluids and tissues).
Equipment
UHPLC-HRMS/MS- AB Sciex X500B
High resolution QTOF mass spectrometer, mass range TOF up to 40kDa, precursor ion selection 5-2250 m/z, TOF-MS resolution is 42000 (FWHM) measured on the (M+6H)6+ charged isotope cluster for bovine insulin at m/z 956, Turbo V Ion source with Twin Sprayer ESI Probe and Twin Sprayer APCI Probe are available, MS/MS acquisition mode both targeted (IDA Information Dependent Acquisition) and untargeted (DIA Data Independent Acquisition).
HPLC-MS/MS LCQ Fleet Thermo Scientific Ion trap mass spectrometer, mass range of 1-2000 Da, ESI and APCI source available, acquisition scan mode: Full Scan, Selected Ion Monitoring (SIM) Selected Reaction Monitoring (SRM)
GC-MS/MS TSQ QUANTUM Thermo Scientific triple quadrupole mass spectrometer mass range 10-1500 Da, EI, CI sources available,acquisition scan mode: Full Scan, Selected Reaction Monitoring (SRM), Selected Ion Monitoring (SIM) Product Ion Scan, Precursor Ion Scan, Neutral Loss Scan
GC-MS DSQII-COMBIPAL Thermo Scientific single quadrupole mass spectrometer mass range of 1 – 1050 Da , EI, CI and DEP (Direct Exposure Probe) sources available, acquisition scan mode: Full Scan, Selected Ion Monitoring (SIM). Combipal autosampler combines Static Headspace, SPME (Solide Phase Micro Extraction) and Liquid Injection
GC-MS DSQII Thermo Scientific, single quadrupole mass spectrometer mass range of 1 – 1050 Da, EI and CI source available, acquisition scan mode: Full Scan, Selected Ion Monitoring (SIM).